Composite

Part:BBa_K3619022:Design

Designed by: Isak Ingerholt   Group: iGEM20_Lund   (2020-09-19)


Fusion Protein GST-EAFP1, Antimicrobial after Enterokinase cleavage


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 177
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is designed and codon optimized for expression in E. coli K12.


Source

This part was created by the iGEM LUND 2020 team based on previous effective AMP production as fusion partners, which can be seen in [1].


References

1. https://pubmed.ncbi.nlm.nih.gov/28807840/